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(A) Purification of GST–Apn2. Eluted GST–Apn2 fusion protein was analyzed on a 7.5% denaturing polyacrylamide gel and stained with Coomassie blue. Lane 1, molecular weight standards; lane 2, 3 µg of eluted GST–Apn2 protein. (B) Kinetics of APE activity of GST–Apn2; lane (–) is the no-protein control. The details are described in Materials and Methods.
