Fig. 10.
Effects of PBEF knock down on IL-8 secretion in HPAEC. A. A representative western blotting image of IL-8, PBEF and β-actin protein detections. HPAEC were treated in the same way as those in A549 cells as described above in Figure 7. Secreted IL-8, cell lysate PBEF and β-actin protein were immunodetected in the same way as described above in Figure 1. B. Quantitation of cell lysate PBEF protein levels by densitometry analysis. C. Quantitation of secreted IL-8 levels by densitometry analysis. Secreted IL-8 and cell lysate PBEF protein levels were quantified by densitometry analyses in the same way as those in A549 cells as described above in Figure 7. **, p<0.01.