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. 2004 Jan 2;32(1):158–168. doi: 10.1093/nar/gkh153

Figure 2.

Figure 2

SDS–PAGE analysis of the representative mutant proteins of the PolDPho complex (A), the small subunit DP1Pho expressed alone (B) and the large subunit DP2Pho (C). The gels (10–15%) were stained with Coomassie Brilliant Blue R-250 after electrophoresis. The protein samples were purified using Hitrap Q, nickel affinity and Superdex-200 columns as described in the Materials and Methods. The band positions of the full-length of the small subunit (DP1Pho) and the large subunit (DP2Pho) are indicated with arrows. Due to the instability of DP2Pho expressed alone, the upper minor band in panel C corresponds to the full-length polypeptide.