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Manganese-dependent ssDNA endonuclease activity of the wild-type PolDPho and the alanine mutants. (A) Degradation of circular ssDNA by the wild-type PolDPho in the presence of manganese. (B) Comparison of the ssDNA endonuclease activities of the alanine mutants. The arrows indicate the bands of the substrates M13 ssDNA.
