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Afadin-dependent recruitment of ZO-1 to nectin-2α–based cell-cell adhesion sites. Nectin-2α-L and -2α-ΔC-L cells were doubly stained with various combinations of the anti–ZO-1 mAb, the anti–nectin-2 mAb, and the anti-afadin mAb. (A) Nectin-2α-L cells. (B) Nectin-2α-ΔC-L cells. Arrows, nectin-2α–based cell-cell adhesion sites; arrowheads, nectin-2α-ΔC–based cell-cell adhesion sites. Bars, 10 μm. The results shown are representative of three independent experiments.
