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. 2004 Jan 9;32(1):287–297. doi: 10.1093/nar/gkh161

Figure 6.

Figure 6

Growth, virulence and adherence assays in trophozoites grown with 5-AzaC. (A) Dose–response analysis of 5-AzaC concentration on the growth of E.histolytica. Data represent the mean and standard deviation of three independent experiments. (B) Cytopathic activity measuring the in vitro ability of amoeba grown in the presence of 5-AzaC (23 µM) to destroy a monolayer of CHO cells. The column +/– shows the cytopathic activity of trophozoites grown for 2 days with 5-AzaC (23 µM) and then cultivated without 5-AzaC for 2 weeks. Data represent the mean and standard deviation of three independent experiments done in duplicate. (C) Adherence ability of trophozoites grown in the presence of 5-AzaC (23 µM) to a formaldehyde-fixed monolayer of CHO cells. Data represent the mean and standard deviation of three independent experiments done in duplicate. (D) Hemolytic assay measuring the ability of trophozoites grown in the presence of 5-AzaC (23 µM) to lyse human red blood cells. Data represent the mean and standard deviation of three independent experiments done in duplicate.