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. 2004 Jan 2;32(1):65–72. doi: 10.1093/nar/gkh166

Figure 1.

Figure 1

Echinomycin blocks chromosomal decondensation and DNA replication in Xenopus sperm nuclei. Demembranated Xenopus sperm chromatin was incubated in Xenopus egg extract for 45 min with (a and b) 6 µM echinomycin or (c and d) solvent. Total DNA was stained using Hoechst 33258 (a and c). Replicated DNA, having incorporated biotinylated dUMP, was stained with streptavidin fluorescein (b and d). (e) Total DNA synthesis was monitored using incorporation of dAMP from [α-32P]dATP in the presence of different concentrations of echinomycin as shown.