| PrimeSTAR buffer (5X) | 10 μl |
| dNTPs (2.5 mM) | 4 μl |
| PCR 5’ primer (10 μM) | 1 μl |
| PCR 3’ primer (10 μM) | 1 μl |
| First strand cDNA (from reverse transcription) | 1-5 μl |
| PrimeSTAR HS DNA polymerase (2.5 units/ μl) | 0.5 μl |
| Sterile water | to 50 μl |
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