RNA (from immunoprecipitation; in water) | 13 μl |
ATP-free T4 RNA ligase buffer (NEB; 10X) | 2 μl |
DMSO | 2 μl |
3’ adapter | 1 μl |
T4 RLN2 (NEB) | 2 μl |
RNA (from immunoprecipitation; in water) | 13 μl |
ATP-free T4 RNA ligase buffer (NEB; 10X) | 2 μl |
DMSO | 2 μl |
3’ adapter | 1 μl |
T4 RLN2 (NEB) | 2 μl |