Figure 5.

Differential distribution of OX₁R and OX₂R mRNA in monoaminergic and cholinergic neurons implicated in regulation of wakefulness. A–D, Coronal brain sections containing TMN (A), DR (B), LC (C), and LDT (D) from wild-type mice were hybridized in situ to an OX₁R (top) or OX₂R (bottom) antisense probe (blue stain) combined with either a monoaminergic marker VMAT2 [histaminergic (A), serotonergic (B), noradrenergic neurons (C)] or cholinergic marker VAChT (D) antisense probe (orange stain). The locations and sizes of magnified images (right) are shown by white rectangles in the low-power images (left). Representative cells stained blue, orange, or both are indicated by blue, orange, and green arrowheads, respectively. Enlargements of some of these representative cells are shown in insets of the left panels. 3v, Third ventricle; 4v, fourth ventricle; Aq, aqueduct. Scale bars: (in A) A–D, 100 and 50 μm for low- and high-power images, respectively.