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Prospore membranes form on opposite spindles in a spo21::GFP strain. Strain AN230 (spo21::GFP/spo21::GFP) was transferred to 2% KOAc and analyzed by fluorescence microscopy as described in MATERIALS AND METHODS. (A) Anti-Ssop staining. (B) Antitubulin staining of the cells in A. (C) DAPI staining of the same cells.
