Table 1.
Effects of SPO21 gene dosage on ascus formation
| Strain | Relevant genotype | Distribution of
ascal types (%)
|
||||
|---|---|---|---|---|---|---|
| % Asci | monad | dyad | triad | tetrad | ||
| AN180 | spo21Δ/spo21Δ | 0 | ||||
| AN231 | spo21∷GFP/spo21Δ | 0 | ||||
| AN230* | spo21∷GFP/spo21∷GFP | 12.6 | 0 | 97 | 3 | 0 |
| AN178* | SPO21/spo21Δ | 83 | 8 | 57 | 0 | 35 |
| AN229* | SPO21/spo21∷GFP | 79 | 1 | 47 | 0 | 52 |
| AN120 | SPO21/SPO21 | 90 | 0 | 19 | 1 | 80 |
Strains of the indicated genotype were scored for sporulation by light microscopy. For comparison of ascal types, at least 500 individual cells were scored.
*
The number of dyads and tetrads observed in strains AN230, AN178, and AN229 are all significantly different (χ2, p < 0.001) from that in AN120.