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Prospore membrane formation in spo21, cnm67, and spo21 cnm67 strains
| Strain | Relevant genotype† | No. of
prospore membranes observed (%)*
|
||||
|---|---|---|---|---|---|---|
| 4 | 3 | 2 | 1 | 0 | ||
| AN120 | SPO21 CNM67 | 75 | 3 | 3 | 0 | 19 |
| AN180 | spo21Δ | 0 | 0 | 0 | 0 | 100 |
| AN161 | cnm67Δ | 16 | 1 | 19 | 0 | 64 |
| AN254 | spo21Δ cnm67Δ | 0 | 0 | 0 | 0 | 100 |
Cells displaying tetranucleate DAPI staining were scored for the number of prospore membranes observed when counterstained with the anti-Sso antibody. At least 135 cells in each culture were counted.
