Figure 6.
Exonuclease and DNA nicking activity of YB-1. (A) Forty nanograms of full-length, mutant YB-1 or p53exo (exonuclease domain of p53) were incubated for 30 min at 37°C as described in Materials and Methods with the different DNA substrates indicated above each gel. Reactions were stopped in the appropriate dye buffer and cleaved DNA products were analyzed on 14% denaturing polyacrylamide gels to analyze the exonuclease activity. (B) Histogram representation of the YB-1 nuclease activity performed in (A) with the duplex DNA substrate. The percentage nuclease activity was calculated by scanning the figures and using the formula: intensities of cleaved products/(intensities of cleaved products + intensity of uncleaved probe) × 100. Results were calculated from duplicate experiments.