Figure 7.
Endonucleolytic activity of YB-1. (A and B) Forty nanograms of full-length, mutant YB-1 or p53exo (exonuclease domain of p53) peptides were incubated with the indicated DNA substrates in reaction buffer for 30 min at 37°C as described in Materials and Methods. DNA products were analyzed on 14% denaturing polyacrylamide gels. The brackets on the right side of each gel indicate the cleavage products of each probe by the proteins. Experiments were repeated twice. (C) Histogram representation of the YB-1 nuclease activity performed in (B). The percentage nuclease activity was calculated by scanning the figures and using the formula: intensities of cleaved products/(intensities of cleaved products + intensity of uncleaved probe) × 100. Results were calculated from duplicate experiments.