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Sensorgrams for the binding of duplexes containing G-Y mismatches to the Npt–Azq immobilized SPR sensor surface. Aliquots of 90 µl of the duplexes (1.0 µM in 10 mM HEPES buffer pH 7.4, 1 M NaCl) containing G-A, G-G mismatch and G-C matches were injected for 180 s to measure the association to the sensor surface.
