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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Hepatology. 2013 Jul 22;58(3):1042–1053. doi: 10.1002/hep.26384

Figure 1. Bioluminescence imaging and histological assessment of EGI-1 cells after xenotransplantation into SCID mice.

Figure 1

High correspondence between the bioluminescence signal in the liver (A) and the macroscopic detection of liver tumors at autopsy (arrow, B) after xenotransplantation of EGI-1 cells into SCID mice by intraportal injection. Before transplantation, EGI-1 cells were transduced with lentiviral vectors encoding the firefly luciferase gene. Mice were sacrificed once the bioluminescence signal intensity in the liver reached a value >105 p/sec/cm2/sr. Histological analysis of liver metastases showed that EGI-1 cells laid embedded in a rich stroma (H&E staining, C). By dual immunofluorescence for EGFP (green) and α-SMA (red), we showed that CAF were strictly adjacent to EGI-1-derived tumors, but coincident labeling between EGFP and α-SMA was never observed (D). In liver tumors formed by EGI-1 cells, FISH showed that α-SMA-positive CAF (green, E, F) co-expressed mouse (red, white arrow, E), but not human (red, F) Y-Chr, which was instead expressed by tumoral EGI-1 cells (F). High specificity of both Y-probes was confirmed in preliminary experiments. Original magnification: C-F, 200x, insets in E,F, 400x.