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. Author manuscript; available in PMC: 2013 Aug 4.
Published in final edited form as: Methods Mol Biol. 2011;769:65–86. doi: 10.1007/978-1-61779-207-6_6

Figure 3. Open imaging chamber used for RBI growth one guidance assay.

Figure 3

(A) Schematic drawing of bottom aluminium plate of custom-made holder used as open imaging chamber. The chamber is open to the top allowing accessing cultured cells via micropipettes. Dimensions are in inches. (B) Brass inset to be mounted on coverslip with cells using high vacuum grease. (C) Assembled open chamber. Brass inset with coverslip attached is positioned into the bottom plate. Metal blades can be used to hold tubing for perfusion of medium. (D) Picture of a fully assembled open chamber.