Skip to main content
. Author manuscript; available in PMC: 2013 Aug 4.
Published in final edited form as: Methods Mol Biol. 2011;769:65–86. doi: 10.1007/978-1-61779-207-6_6

Figure 4. Restrained bead interaction assay.

Figure 4

(A) DIC image of an Aplysia growth cone on poly-lysine substrate. Dashed line indicates the central domain boundary. (B) The same growth cone is shown shortly after an apCAM-coated bead is positioned onto the peripheral domain close to the leading edge and restrained with a microneedle against retrograde translocation. This image is taken at the beginning of latency phase during which the coupling between apCAM and F-actin is weak. (C) As coupling strength increases, the growth cone undergoes rearrangements, such as central domain extension towards the bead and leading edge growth in front of the bead. Scale bar: 10 µm.