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. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: Cancer Res. 2013 Mar 27;73(10):3041–3050. doi: 10.1158/0008-5472.CAN-12-3947

Figure 4.

Figure 4

HMGA2 regulates IGF2BP2, which is necessary for ERMS growth. A, heatmap of microarray on HMGA2 knockdown samples illustrates HMGA2-regulated genes in rhabdomyosarcoma cells. B, genes significantly downregulated by HMGA2 knockdown are enriched for those controlling cell death, growth, and proliferation, including NRAS. Gene sets were analyzed by IPA software. C, knockdown of HMGA2 reduces IGF2BP2 mRNA. *, P < 0.05, Student t test, sh-HMGA2 sample versus sh-NT sample. D, knockdown of HMGA2 reduces IGF2BP2 protein. E, HMGA2 and IGF2BP2 mRNA levels are correlated in cancer cell lines. F, knockdown of IGF2BP2 using lentiviral-based shRNAs. **, P < 0.01, Student t test, sh-IGF2BP2 sample versus sh-NT sample. G, knockdown of IGF2BP2 in ERMS cells reduces proliferation and induces cell death. Images were taken 96 hours after infection. Scale bar, 100 μm. H, knockdown of IGF2BP2 using doxycycline-inducible shRNAs in rhabdomyosarcoma cells inhibits colony formation.

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