Skip to main content
. 2004 Jan 16;32(1):386–396. doi: 10.1093/nar/gkh174

Figure 4.

Figure 4

Northern blot analyses of total RNA from R.capsulatus B10 cells containing plasmid pBBRgroΔCR or pBBRgroΔCRΔCIRCE. (A) Induction of 0.23 kb groΔCR and (B) 0.22 kb groΔCRΔCIRCE transcript following heat shock at 39°C. RNA was isolated from R.capsulatus B10 containing plasmid pBBRgroΔCR or plasmid pBBRgroΔCRΔCIRCE grown at 32°C (0 min) and at the indicated times after a temperature shift to 39°C. gro- specific transcripts were monitored by northern hybridization using the groES-specific 0.1 kb probe. The mRNA amount was determined as described and plotted as a function of time.