Figure 4.

The 5′ UNA sense strand modification improves strand selection for U5-targeted siRNA and dsiRNA. (a) Design of three siRNAs within U5 reporter target sequence. The black bar indicates the span of the AS strand for each of the indicated siRNAs. (b) Dual luciferase gene-silencing potency of U5-targeted siRNAs. (c) Schematic (shown above each graph) and dual luciferase assays gene-silencing performance of 5′ UNA siU5-3 (UsiU5-3), unmodified dsiU5-3, and 5′ UNA dsiU5-3 (UdsiU5-3). IC₅₀ values for each strand and AS:S IC₅₀ ratios are shown in the insets. n ≥ 3, in duplicate, mean ± SEM.