Skip to main content
. 2004 Jan 16;32(1):371–379. doi: 10.1093/nar/gkh181

Figure 3.

Figure 3

G-quadruplex stabilization of sequences from the hTERT intron 6. (a) Schematic representation of the –β deletion of hTERT that encompassed intron 6, exon 7, intron 7, exon 8 and intron 8 according to Leem et al. (17). G4TERT1 and G4TERT2 are located in the 5′ part of intron 6. VNTR6-1 represented a fragment of a 38 base repeat that extended 1.0–1.8 kb in the mid-part of intron 6. (b) UV melting behavior of G4TERT1, G4TERT2 and VNTR6-1 oligonucleotides and mutated sequences (3 µM) in 10 mM sodium cacodylate buffer (pH 7.0) and 100 mM KCl, NaCl or LiCl, as indicated. Denaturation of the quadruplexes is shown by a decrease in absorbance at 295 nm (12). G4TERT1 in KCl, –0.01 offset; G4TERTa in LiCl, –0.004 offset; G4TERT2 in KCl, –0.01 offset; G4TERT2mu in NaCl, –0.03; G4TERT1mu in NaCl, –0.017.