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. 2004 Jan 16;32(1):371–379. doi: 10.1093/nar/gkh181

Figure 4.

Figure 4

Effect of 12459 on the formation of hTERT intron 6 quadruplex by a PCR-stop assay. (a) Principle of the assay. Test oligomer was amplified with a complementary oligomer overlapping the last G-repeat. Taq polymerase extension resulted in the formation of a final double-stranded PCR product. In the presence of a ligand that stabilizes oligomer into a G-quadruplex structure, annealing of oligomer and, therefore, Taq polymerase extension is inhibited. Note that the diagram represents a G-quadruplex formed with three G-quartets, as an example. (b) Effect of increasing concentrations of 12459 on the PCR-stop assay with G-quadruplex-forming oligomers (G4TERT1, G4TERT2 and VNTR6-1) or with control mutated oligomers (G4TERT1mu, G4TERT2mu and VNTR6-1mu). The sequence of oligomers is indicated in Table 1.