Fig. 5.

The effect of decapitation by neck ligation of CAX larvae on the timing of their transition to the wandering stage. (A) The experimental protocol was as follows: newly ecdysed CAX larvae were starved for 2.5 d, fed for 24 h to start their molt timers, and then neck-ligatured at various times after they initiated feeding. (B) Larvae neck-ligatured a day after being given food showed delayed signs of wandering, but those ligated later showed a timing equivalent to the CAX larvae that had just been given the 1-d feeding bout. Bars give the mean and SEM for each group. N is the number of animals in each group.