Fig. 3.

Phosphorylation of serine residues adjacent to the inhibitory peptide is important for Atg29 function. (A) Pho8Δ60 WT (TN124) cells were transformed with empty vector; Pho8Δ60 atg29∆ (YIY36) cells were transformed with empty vector or a plasmid containing WT or different mutant forms of Atg29-PA as indicated. Cells were cultured in SMD (+N) to midlog phase and shifted to SD-N for 4 h. Protein extracts were analyzed using the Pho8Δ60 assay. Error bars correspond to the SD and were obtained from three independent repeats. (B and C) Relative activities of different Atg29 mutants based on the results in A.