The Rca system regulates iflA mRNA and IflA protein abundance. (A) Genomic location of iflA and L box (small black arrow). Upper arrows, transcript locations/sizes. (B) iflA L box sequence, underlined; direct repeat, bold; transcription start site, bent arrow. (C) Histogram showing mean iflA transcript levels for strains and light colors indicated (G, green light; R, red light), normalized using the 16S ribosomal values for each lane. Error bars represent SEM. (D) Representative immunoblot showing IflA abundance during growth in the light colors indicated in C.