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ITC data for the titration of HG to wild-type (A) and Y346F (B) HGDOPp. (Upper) The binding isotherms with exothermic heat change. (Lower) The fits of the integrated enthalpic changes to a one-site binding model. Dissociation constants (Kd) were determined to be 15.8 and 909 µM for wild-type and Y346F variant of HGDOPp, respectively.
