Fig. 2.

TLR-4/MyD88 knockdown in mice reduces caloric intake, but does not directly protect mice from saturated fat-induced defects in hepatic insulin signaling. Mice treated with ASOs against either TLR-4 or MyD88 were protected against hepatic triglyceride deposition (A) when fed a diet rich in saturated fat because of a decreased caloric intake (B). However, TLR-4/MyD88 knockdown did not protect mice from hepatic triglyceride accumulation (C), membrane translocation of PKCε (D), and impairment of insulin-stimulated Akt2 (E) and FoxO1 (F) phosphorylation following lipid gavage with lard. n = 5–10 per group. *P < 0.05. Con, gavaged control.