Immunofluorescence localization of
PHOsh1 domain to the TGN.
Δosh1 yeast (TLY221) expressing
GFP-tagged PHOsh1 and the helical domain (residues
280–690, pTL327) were fixed and stained with rabbit antibodies to
Tlg1p (A), Anp1p (B), and Pep12p (C), markers for TGN/early endosomes,
cis-Golgi, and prevacuolar compartment, respectively.
Colocalization of GFP is only seen with Tlg1p (arrowheads), but not
with the other two markers (arrows).