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. 2001 Jun;12(6):1633–1644. doi: 10.1091/mbc.12.6.1633

Figure 7.

Figure 7

Immunofluorescence localization of PHOsh1 domain to the TGN. Δosh1 yeast (TLY221) expressing GFP-tagged PHOsh1 and the helical domain (residues 280–690, pTL327) were fixed and stained with rabbit antibodies to Tlg1p (A), Anp1p (B), and Pep12p (C), markers for TGN/early endosomes, cis-Golgi, and prevacuolar compartment, respectively. Colocalization of GFP is only seen with Tlg1p (arrowheads), but not with the other two markers (arrows).