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Binding site preference for distamycin. (A) Structure of distamycin. (B) Selection oligonucleotide with the sequence of N(9)AA·UU was incubated with 0, 50, 150, 450 or 1350 nM distamycin for 2 h, followed by digestion with UDG and then PCR amplification. PCR product was then purified and sequenced. Positions relative to the AA·UU are indicated at the bottom of each panel by –1 to –9. Black, G; blue, C; green, A; red, T.
