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. 2004 Jan 12;32(1):e9. doi: 10.1093/nar/gng157

Figure 4.

Figure 4

Figure 4

Figure 4

Figure 4

Specificity, kinetics and efficiency of hybridization on PMMA. The aminated PMMA was obtained by the chemistry presented in this report followed by cross-linking with glutaraldehyde. The 5′-aminated probes were immobilized for 2 h, washed and hybridized with 0.2 µM target solution for 2 h. (A) Specificity of DNA probes was tested by hybridization with a complementary (closed circles) and with a non-complementary (open circles) DNA target solution. (B) The kinetics of DNA target hybridization to micorarrays on PMMA. (C) Rate of hybridization for different densities of immobilized DNA probes on aminated PMMA slides. The rate of hybridization is determined from the linear part of the kinetic curves in (B). (D) Hybridization efficiency of amino-labeled DNA probes on PMMA when hybridized to a complementary strand (target-Cy5), varying the hybridization time and the amount of covalently immobilized probes. Efficiency of hybridization was calculated as the fraction of covalently immobilized DNA, which participated in duplex formation with the complementary DNA target.