Table 3. Sequence of oligonucleotides used.
DNA probe | Sequence (5′–3′) | 5′ End | 3′ End |
---|---|---|---|
Target-Cy5 | CGT CAT CGG GCT TTG CAT | Cy5 | None |
Probe-NH2 | ATG CAA AGC CCG ATG ACG | C6-NH2 | Cy3 |
Probe-SH | ATG CAA AGC CCG ATG ACG | SH | Cy3 |
Probe-Cy3 | ATG CAA AGC CCG ATG ACG | None | Cy3 |