Fig. 5.
WNV genomic and subgenomic RNAs do not induce an antiviral response. Cells were transfected with ISG56-luc and pCMV-Renilla as described in Fig. 1. Cultures were subsequently transfected with 500 ng of: (A) genomic RNA isolated from culture supernatants (FL-WNV) or full length in vitro transcribed and capped WNV genomic RNA (capped-FL-IT-WNV) or (B) the indicated sfRNA constructs. Luciferase expression was assessed as described in Fig. 1 the indicated sfRNA constructs. Luciferase expression was assessed as described in Fig. 1.