Table 2.
Plasmids used in this study
| Name | Description | Source |
|---|---|---|
| pGEM-5Z | In vitro transcription vector | Promega |
| pBAT4 | In vitro transcription vector | Peranen et al. (1996) |
| pGEX-4T-1 | GST expression vector (E. coli) | Amersham Pharmacia Biotech |
| pGEX-3X | GST expression vector (E. coli) | Amersham Pharmacia Biotech |
| YEpLG-GST | 2 μm LEU2 GAL1 promoter GST expression vector (yeast) | Shulewitz et al. (1999) |
| pACT | Two-hybrid Gal4(TAD) expression vector | Durfee et al. (1993) |
| YCplac111 | CEN LEU2 | Gietz et al. (1988) |
| YCplac22 | CEN TRP1 | Gietz et al. (1988) |
| YCpUG | CEN URA3 GAL1 | Bardwell et al. (1998) |
| YCpLG | CEN LEU2 GAL1 | Bardwell et al. (1998) |
| pGEM-5Z-HSL7 | pGEM-5Z +HSL7 | This study |
| pBAT4-HSL7(1–246) | pBAT4 +HSL7(1–246) | This study |
| pBAT4-HSL7(88–544) | pBAT4 +HSL7(88–544) | This study |
| pBAT4-HSL7(168–345) | pBAT4 +HSL7(168–345) | This study |
| pBAT4-HSL7(316–636) | pBAT4 +HSL7(316–636) | This study |
| pGEX-HSL7 | pGEX-4T-1 +HSL7 | This study |
| YEpLG-GST-HSL7(1–685) | YEpLG-GST +HSL7(1–685) | This study |
| YEpLG-GST-HSL7(1–246) | YEpLG-GST +HSL7(1–246) | This study |
| YEpLG-GST-HSL7(316–636) | YEpLG-GST +HSL7(316–636) | This study |
| pGEX-HSL7(674–827) | pGEX-4T-1 +HSL7(674–827) | This study |
| pGEX-HSL7(674–736) | pGEX-4T-1 +HSL7(674–736) | This study |
| pGEX-HSL7(737–827) | pGEX-4T-1 +HSL7(737–827) | This study |
| pGEX-HSL7(771–827) | pGEX-4T-1 +HSL7(771–827) | This study |
| pGEX-SWE1 | pGEX-3X +SWE1 | This study |
| pGEX-SWE1(284–370) | pGEX-4T-1 +SWE1(284–370) | This study |
| pGEX-HSL1ΔN | pGEX-4T-1 +HSL1(833–1518) | This study |
| pGEX-HSL1ΔNΔC | pGEX-4T-1 +HSL1(1018–1244; 1482–1518) | This study |
| YCpT-ADHp-GAL4(DBD)-HSL7 | Gal4(DBD)-Hsl7 production from ADH1 promoter on a CEN TRP1 plasmid | This study |
| YCpT-ADHp-GAL4(DBD)-HSL7(Δ224–392) | Gal4(DBD)-Hsl7(Δ224–392) production from ADH1 promoter on a CEN TRP1 plasmid | This study |
| YCpT-ADHp-GAL4(DBD)-HSL7(352–827) | Gal4(DBD)-Hsl7(352–827) production from ADH1 promoter on a CEN TRP1 plasmid | This study |
| YCpT-ADHp-GAL4(DBD)-HSL7(224–827) | Gal4(DBD)-Hsl7(224–827) production from ADH1 promoter on a CEN TRP1 plasmid | This study |
| YCpT-ADHp-GAL4(DBD)-HSL7(284–827) | Gal4(DBD)-Hsl7(284–827) production from ADH1 promoter on CEN TRP1 plasmid | This study |
| YCpT-ADHp-GAL4(DBD)-HSL7(1–533) | Gal4(DBD)-Hsl7(1–533) production from ADH1 promoter on CEN TRP1 plasmid | This study |
| pACT-HSL1(987–1518) | pACT +HSL1(987–1518) | Shulewitz et al. (1999) |
| pACT-SWE1(295–819) | pACT +SWE1(295–819) | Shulewitz et al. (1999) |
| YCpL-HSL7 | YCplac111 +HSL7 | This study |
| YCpL-HSL7(V251A) | YCplac111 +HSL7(V251A) | This study |
| YCpL-HSL7(P250Y) | YCplac111 +HSL7(P250Y) | This study |
| YCpL-HSL7(K254E) | YCplac111 +HSL7(K254E) | This study |
| YCpL-HSL7(F242L) | YCplac111 +HSL7(F242L) | This study |
| YCpUG-HSL7 | YCpUG +HSL7 | Shulewitz et al. (1999) |
| YCpUG-HSL7-Myc | YCpUG +HSL7-Myc | Shulewitz et al. (1999) |
| YCpT-GFP-HSL7 | YCplac22 +GFP-HSL7 | Shulewitz et al. (1999) |
| YCpLG-GFP-HSL7 | YCpLG +GFP-HSL7 | Shulewitz et al. (1999) |
| YCpT-GFP-HSL7(V251A) | YCplac22 +GFP-HSL7(V251A) | This study |
| YCpT-GFP-HSL7(P250Y) | YCplac22 +GFP-HSL7(P250Y) | This study |
| YCpT-GFP-HSL7(K254E) | YCplac22 +GFP-HSL7(K254E) | This study |
| YCpT-GFP-HSL7(F242L) | YCplac22 +GFP-HSL7(F242L) | This study |
| YCpT-GFP-HSL7(1–685) | YCplac22 +HSL7(1–685) | This study |
| YCpLG-HSL1(HA)3 | YCpLG +HSL1(HA)3 | Shulewitz et al. (1999) |
| YCpLG-HSL1-K110R(HA)3 | YCpLG +HSL1-K110R(HA)3 | This study |