Table 2. rAAV component sizes.
| rAAV-Neo-YFG | rAAV-Hyg-YFG | ||
|---|---|---|---|
| Fragment | Size (bp) | Fragment | Size (bp) |
| L-ITR | 141 | L-ITR | 141 |
| Left homology arm | 900 | Left homology arm | 900 |
| Linker A | 23 | Linker A | 23 |
| 5′ loxP to Linker A | 11 | 5′ loxP to Linker A | 13 |
| 5′ loxP | 34 | 5′ loxP | 34 |
| PGK | 529 | PGK | 513 |
| Neo + pA | 1091 | Hyg B + pA | 1603 |
| EM7 + Zeo | 453 | EM7 + Zeo | 453 |
| 3′ loxP | 34 | 3′ loxP | 34 |
| 3′ loxP to Linker B | 10 | 3′ loxP to Linker B | 10 |
| Linker B | 23 | Linker B | 23 |
| Right homology arm | 900 | Right homology arm | 900 |
| R-ITR | 141 | R-ITR | 141 |
| Total | 4290 | Total | 4788 |