Figure 5. Fibrocyte transfer modulates systemic cytokines.
At the time of CLP surgery, fibrocytes or an equal volume of saline were transferred to the abdomen of C57BL/6 mice. The animals were euthanized at the early time points of 12, 24, and 48 hours and blood collected from the retroorbital sinus for cytokine analysis with ELISAs for A) IL-6, B) IL-10, C) IFN-γ, and D)IL-4. Data are reported as mean ± SEM. n= 6–9/group with 3 independent experiments, IL-6 24 hour groups n=20, 4 independent runs. * = p<0.005 as compared to saline group at that time point. Brackets (black = Fibrocyte, grey = Saline) indicate significance over time within a treatment group.