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. Author manuscript; available in PMC: 2013 Aug 5.
Published in final edited form as: Cell. 2009 Feb 6;136(3):521–534. doi: 10.1016/j.cell.2008.11.044

Figure 7.

Figure 7

L-Glutamine and SLC1A5 Regulate Autophagy

(A) Confocal images of mCherry-eGFP-LC3a distribution before (+) and after (−) L-glutamine starvation are shown.

(B) Quantitation of autophagy flux in RT112 cells.

(C) RT112 cells transfected with the individual siRNAs for 72 hr were serum starved for 24 hr and processed for autophagy flux quantitation. Rapamycin was added to scrambled siRNA transfected cells during the starvation period.

(D) EAA regulation of mTOR signaling, cell growth and suppression of autophagy requires intracellular uptake and subsequent efflux of L-glutamine (SLC3A2 dimerized with SLC7A5 is shown in yellow).

All data (mean ± SD) are representative of at least three independent experiments.