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. Author manuscript; available in PMC: 2013 Aug 5.
Published in final edited form as: J Invest Dermatol. 2010 Feb 25;130(6):1558–1570. doi: 10.1038/jid.2010.25

Figure 2.

Figure 2

The number of mast cells, eosinophils, CD4+ T cells, CD8+ T cells, and macrophages in the skin from wild type mice (WT), L-selectin−/− mice (L−/−), ICAM-1−/− mice (I−/−), and L-selectin/ICAM-1−/− mice (L/I−/−) treated with either DNFB (A) or FITC (B) on the ears before and 24 hours (h) after challenge. Mast cells were identified by toluidine blue staining, whereas eosinophils were identified by H&E staining. Macrophages, CD4+ T cells, and CD8+ T cells were stained with F4/80, anti-CD4 mAb, and anti-CD8 mAb, respectively. The number of cells was counted in 10 random grids under magnification of ×400 high-power fields (HPF) and averaged. Each histogram shows the mean (± SEM) results obtained from 6 mice of each group. †p<0.05 vs. before elicitation in each group. *p<0.05 and **p<0.01.