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NEIL1’s C-terminus provides CID for its partner proteins. Far Western analysis with purified proteins (A), and FLAG co-IP analysis in HEK293 cell extracts expressing FLAG-WT NEIL1 or the FLAG-N311 mutant (B) show the requirement of the CID for NEIL1’s interactions with downstream SN-BER proteins. (C) PLA analysis confirms in-cell association of SN-BER proteins in the nucleus with ectopic WT NEIL1 but not the N311 mutant. Other details are in the Experimental Section.
