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The lack of NEIL1’s CID sensitizes HEK293 cells to oxidative stress. (A) Western analysis of NEIL1 levels after its depletion by 3'-UTR specific siRNA in HEK293 cells and simultaneous expression of FLAG-WT NEIL1 or FLAG-N311 mutant polypeptide. (B) Survival of HEK293 cells transfected with control siRNA or siRNA for NEIL1, and simultaneous co-transfection of FLAG-WT NEIL1 or FLAG-N311 mutant expression plasmids. The details are in the Experimental Section.
