Table 2. Anabaena circinalis 131C putative specific sequences with protein matches in The National Center for Biotechnology Information (NCBI) protein database.

ID	Best BLASTX hit	Organism	% Identity	% Similarity	Microarray hybridization	Specificitya
SSH
65	Hypothetical protein	Cytophaga hutchinsonii	57	81	5.3 ± 0	+
70	TonB, ferric-siderophore uptake	B.thetaiotaomicron VPI-5482	42	66	4.9 ± 0.3	+
73	Alpha-amylase precursor	C.acetobutylicum	47	58	4.6 ± 2.6	+
71	NADH2 dehydrogenase	C.elongatum	70	75	4.4 ± 0.7	+
175	GLP_291_11778_8566	G.lamblia ATCC 50803	29	43	4.5 ± 0.8	+
75	No similarity				3.9 ± 1.8	+
74	Hypothetical protein	Neurosporra crassa	46	66	3.6 ± 1.9	+
69	Hypothetical protein	B.japonicum USDA 110	62	83	2.9 ± 0	+
77	Sulfatase family protein	N.aromaticivorans	42	57	2.8 ± 0.5	+
68	Hypothetical protein	Naromaticivorans	91	95	2.7 ± 0.2	+
72	Putative integral membrane protein	S.coelicolor A3(2)	46	61	2.6 ± 0.5	+
Subtracted PPH
104	Hypothetical protein, transcriptase	Caenorhabditis elegans	32	48	6.8 ± 0.3	+
95	Putative hydrolase	B.thetaiotaomicron VPI-5482	53	67	4.1 ± 0.3	+
185	Novel antigenic to ORF2	T.orientalis	94	94	1.8 ± 0	≈
120	Hypothetical protein	Nostoc sp. PCC7120	87	90	1.7 ± 0.3	≈
119	50S ribosomal protein L33	P.lunula	80	96	1.6 ± 0.1	≈
99	Hypothetical protein	N.punctiforme	63	80	1.4 ± 0.5	≈
186	Thiamin-phosphate pyrophosphorylase	Nostoc sp. PCC7120	70	81	1.2 ± 0.4	≈
98	RNA polymerase ECF-type sigma factor	B.thetaiotaomicron VPI-5482	38	61	1.1 ± 0	≈
102	Unknown protein	Nostoc sp. PCC7120	80	100	0.9 ± 0.2	≈
97	Putative gluconate aldolase	Microscilla sp. PRE1	33	56	ND	ND
106	Hypothetical protein	C.hutchinsonii	67	72	ND	ND
187	ABC transporter ATP-binding	B.thetaiotaomicron VPI-5482	60	70	ND	ND
189	Phosphoglucomutase–phosphomannomutase	C.hutchinsonii	58	69	ND	ND
Unsubtracted PPH
108	Hypothetical protein	C.hutchinsonii	41	61	6.6 ± 1.9	+
179	Putative transposase	Synechocystis sp. BO8402	35	60	5.6 ± 1.1	+
109	Hypothetical protein	B.thetaiotaomicron VPI-5482	23	43	4.9 ± 0.3	+
112	Hypothetical protein	T.erythraeum IMS101	44	65	3.5 ± 1.8	+
115	No similarity				3.4 ± 0.2	+
111	Cell division GTPase	C.hutchinsonii	56	72	2.4 ± 0.8	+
107	No similarity				2.4 ± 0	+
183	Acetyltransferase	B.thetaiotaomicron VPI-5482	60	82	2.4 ± 0.7	+
178	Hypothetical protein	T.fusca	35	57	2.1 ± 0.4	+
118	60 kDa chaperonin GroEL	C.hutchinsonii	91	100	2 ± 0.4	+
110	Hypothetical protein, putative protease	C.hutchinsonii	35	65	ND	ND
180	DNA polymerase III alpha subunit	B.thetaiotaomicron VPI-5482	78	84	ND	ND
182	Transport protein	X.axonopodis pv. citri 306	28	46	ND	ND

^aToxic-strain specificity.

+, Toxic-strain specific; ≈, unspecific; ND, not detected.

Microarray experiments represent hybridizations between strains 131C (Cy5) and 306A (Cy3) using genomic DNA. Microarray values are expressed as average ± standard error of Cy5:Cy3 normalized ratios. Toxic- and non-toxic-strain specificity levels are defined by Cy5:Cy3 ratios >2 and <0.5, respectively.