Table 3. Anabaena circinalis 344B putative specific sequences with protein matches (Id: identity; Sim: similarity) in the NCBI protein database.

ID	Best BLASTX hit	Organism	% Identity	% Similarity	Microarray hybridization	Specificitya
SSH
91	Magnesium chelatase, subunit I	B.thetaiotaomicron VPI-5482	55	84	8.7 ± 0.2	+
89	Excinuclease ABC subunit A	C.hutchinsonii	47	66	7.6 ± 1.3	+
88	50S ribosomal protein L33	P.lunula	78	93	6.8 ± 0.6	+
173	Hypothetical protein	N.aromaticivorans	61	73	6.2 ± 0.2	+
84	Penicillin-binding protein	Bacillus subtilis	51	59	4.9 ± 0.1	+
90	Hypothetical protein	B.thetaiotaomicron VPI-5482	36	59	4.1 ± 0	+
85	Hypothetical protein	Arabidopsis thaliana	56	77	4 ± 0.1	+
86	Similar to chloride channel	N.punctiforme	73	85	2.3 ± 0	+
87	Thiamin-phosphate pyrophosphorylase	Nostoc sp. PCC7120	77	89	0.8 ± 0.3	≈
170	Unknown protein	Oriza sativa	28	41	ND	ND
174	Putative hydrolase	B.thetaiotaomicron VPI-5482	48	67	ND	ND
Subtracted PPH
95	Putative hydrolase	B.thetaiotaomicron VPI-5482	53	67	8.2 ± 0.7	+
119	50S ribosomal protein L33	P.lunula	80	96	6.2 ± 0.6	+
98	RNA polymerase ECF-type sigma factor	B.thetaiotaomicron VPI-5482	38	61	5.9 ± 0.4	+
104	Hypothetical protein, transcriptase	C.elegans	32	48	4.1 ± 0.3	+
106	Hypothetical protein	C.hutchinsonii	67	72	4.1 ± 0.1	+
185	Novel antigenic ToORF2	T.orientalis	94	94	2.2 ± 0.2	+
99	Hypothetical protein	N.punctiforme	63	80	1.3 ± 0.1	≈
186	Thiamin-phosphate pyrophosphorylase	Nostoc sp. PCC7120	70	81	0.8 ± 0.2	≈
102	Unknown protein	Nostoc sp. PCC7120	80	100	0.7 ± 0	≈
97	Putative gluconate aldolase	Microscilla sp. PRE1	33	56	ND	ND
120	Hypothetical protein	Nostoc sp. PCC7120	87	90	ND	ND
187	ABC transporter ATP-binding	B.thetaiotaomicron VPI-5482	60	70	ND	ND
189	Phosphoglucomutase–phosphomannomutase	C.hutchinsonii	58	69	ND	ND
Unsubtracted PPH
179	Putative transposase	Synechocystis sp. BO8402	35	60	22.6 ± 2	+
108	Hypothetical protein	C.hutchinsonii	41	61	18.7 ± 0.4	+
107	No similarity				13.8 ± 0.7	+
111	Cell division GTPase	C.hutchinsonii	56	72	11.6 ± 1.7	+
178	Hypothetical protein	T.fusca	35	57	11 ± 1.9	+
109	Hypothetical protein	B.thetaiotaomicron VPI-5482	23	43	8.6 ± 0.1	+
115	No similarity				6.6 ± 1.6	+
182	Transport protein	X.axonopodis pv. citri 306	28	46	6.5 ± 1.2	+
183	Acetyltransferase	B.thetaiotaomicron VPI-5482	60	82	1.6 ± 0.3	≈
110	Hypothetical protein	C.hutchinsonii	35	65	ND	ND
112	Hypothetical protein	T.erythraeum IMS101	44	65	ND	ND
118	60 kDa chaperonin GroEL	C.hutchinsonii	91	100	ND	ND
180	DNA polymerase III alpha subunit	B.thetaiotaomicron VPI-5482	78	84	ND	ND

^aToxic-strain specificity.

+, Toxic-strain specific; ≈, unspecific; ND, not detected.

Genomic DNA experiments represent microarray hybridizations between strains 344B (Cy5) and 271C (Cy3). Microarray values are expressed as in Table 2.