Fig. 12.

Levels of p24 proteins in knock-out (KO) mutants or amiRNA lines. Western blot analysis with antibodies against the N-terminus of p24δ5, p24δ9, or p24β3, or the C-terminus of p24β2 in membranes from the wild type (Col-0) or the indicated KO mutants or amiRNA lines (see text for details). The expected positions for p24δ5/δ9, p24β3, and p24β2 (according to the western blot analysis shown in Fig. 1) are shown by arrowheads. Molecular weight markers are indicated on the left. A 25 μg aliquot of protein was loaded in each lane. Western blotting with an antibody against the plasma membrane (PM) ATPase was used as a loading control. Lower panel shows a quantification of the levels of each of the proteins in each mutant calculated as a percentage of the levels present in wild-type (Col-0) membranes (mean±SD from three independent experiments).