Table 1.
Co-localization of RFP–p24δ5/δ9 and GFP–p24β2/β3 in co-expression experiments
| A. | ||||
|---|---|---|---|---|
| Combination of proteins | Manders coefficient | |||
| A | B | M1 (A overlapping with B) | M2 (B overlapping with A) | |
| GFP–p24β2 | RFP–p24δ9 | 0.84±0.05 | 0.36±0.07 | |
| GFP–p24β3 | RFP–p24δ9 | 0.47±0.10 | 0.14±0.06 | |
| GFP–p24β3 | RFP–p24δ5 | 0.45±0.07 | 0.15±0.06 | |
| B. | ||||
| Combination of proteins | Co-localization (%) | |||
| RFP–p24δ5 | RFP–p24δ9 | YFP–p24β3 | CFP–p24β2 | 9.19±2.64 |
| RFP–p24δ5 | RFP–p24δ9 | YFP–p24β3 | – | 16.06±2.82 |
| RFP–p24δ5 | RFP–p24δ9 | – | CFP–p24β2 | 30.31±3.71 |
| – | – | YFP–p24β3 | CFP–p24β2 | 44.44±3.31 |
In A, measurements were made on 10 separate cells upon double co-expression (Fig. 7), and calculated with ImageJ 1.47i and the plugins JACoP (Bolte S, Cordelieres FP 2006) and PSC Colocalization (French et al., 2008).