Table 2.
Levels of p24β2 and p24β3 in co-expression experiments
| Expression conditions | Intensity (arbitrary units) | ||
|---|---|---|---|
| p24β2 | GFP | p24β3 | |
| –DNA | 0.0 | 0.0 | 0.0 |
| p24β2 | 6.8 | 6.9 | 0.1 |
| p24β3 | 0.3 | 11.0 | 10.7 |
| p24β2+p24β3 | 7.0 | 17.0 | 10.0 |
| p24β2+p24δ5 | 27.0 | 27.2 | 0.2 |
| p24β2+p24δ9 | 12.0 | 12.1 | 0.1 |
| p24β3+p24δ5 | 0.5 | 17.0 | 16.5 |
| p24β3+p24δ9 | 0.3 | 24.0 | 23.7 |
| p24β2+p24β3+p24δ5 | 18.0 | 34.1 | 16.1 |
| p24β2+p24β3+p24δ9 | 16.0 | 36.2 | 20.2 |
| p24β2+p24β3+p24δ5+p24δ9 | 12.1 | 52.8 | 40.7 |
Quantification of western blots from two different co-expression experiments like the one shown in Fig. 11, using the Quantity One software (Bio-Rad Laboratories). The amount of p24β3 was calculated as the difference between the intensity of GFP (which includes the signal of both GFP–p24β2 and GFP–p24β3) and that of p24β2. Western blots in the linear range of detection that showed comparable intensities for p24β2 and GFP in the co-expression of p24β2+p24δ5 and p24β2+p24δ9 were selected.