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. 2004 Jan 22;32(2):432–440. doi: 10.1093/nar/gkh158

Figure 2.

Figure 2

Oligonucleotide sequences. a, 2-aminopurine, (2AP); F, 6MI pteridine. Triplex-forming oligonucleotides: RCW, positive control for the triplex with no fluorophore; R2APCW is the 2AP-containing triplex with a -GT4C- loop connecting the third strand to the CW duplex; R2APCW-2 is the 2AP-containing triplex with a -CT4G- loop connecting the third strand to the CW duplex; R6MICW, 6MI-containig triplex; NCW, non-triplex-forming control with no fluorophore; N2APCW, 2AP-containing non-triplex-forming control. Hairpin-forming oligonucleotides: R2APC, hairpin designed for detecting the fluorescence signal of the 2AP base analog involved in the 2AP*T base pair; CW, the hairpin representing the duplex part of the triplex; N2APC, non-duplex-forming control. Single-stranded control oligonucleotides: R2AP, R6MI and N2AP.