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. 2004 Jan 22;32(2):432–440. doi: 10.1093/nar/gkh158

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Temperature dependence of intensity of the fluorescence emission at 370 nm of 2AP, incorporated in the oligonucleotides. The excitation wavelength was 310 nm. (A) oligonucleotides R^2APCW (filled circles), R^2APC (filled triangles) and R^2AP (crosses); (B) control N^2APCW (filled circles), ds N^2APC (filled triangles), ss N^2AP (crosses); (C) oligonucleotides R^2APCW-2 (filled circles) and N^2APCW (crosses). Solid curves are the best theoretical fits (see Materials and Methods); dashed curves are the smoothed experimental data. Derived thermodynamic parameters are given in Table 1. Solution conditions as in Figure 4.

Temperature dependence of intensity of the fluorescence emission at 370 nm of 2AP, incorporated in the oligonucleotides. The excitation wavelength was 310 nm. (A) oligonucleotides R^2APCW (filled circles), R^2APC (filled triangles) and R^2AP (crosses); (B) control N^2APCW (filled circles), ds N^2APC (filled triangles), ss N^2AP (crosses); (C) oligonucleotides R^2APCW-2 (filled circles) and N^2APCW (crosses). Solid curves are the best theoretical fits (see Materials and Methods); dashed curves are the smoothed experimental data. Derived thermodynamic parameters are given in Table 1. Solution conditions as in Figure 4.