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Temperature dependence of intensity of the fluorescence emission at 430 nm of 6MI, incorporated into the oligonucleotides. The excitation wavelength was 350 nm. The oligonucleotides were R6MICW (filled circles) and R6MI (crosses); solid curves are the best theoretical fits (see Materials and Methods). Derived thermodynamic parameters are given in the Table 1. Solution conditions as in Figure 4.
