Fig. 9.

Identification of nucleases in quinoa perisperm tissue undergoing programmed cell death. Total proteins (50 μg of protein) were extracted from different stages (3–7) of quinoa grain development and were analysed by in-gel nuclease assay at the indicated pH. Nucleases active in the presence of Mg²⁺ and Ca²⁺ (left panel), and of Zn²⁺ (right panel). Double-stranded DNA was used as a substrate.